ABSTRACT The central nervous system is a dense, layered, 3D interconnected network of populations of neurons,and thus recapitulating that complexity for in vitro CNS models requires methods that can createdefined topologically-complex neuronal networks. Several three-dimensional patterning approacheshave been developed but none have demonstrated the ability to control the connections betweenpopulations of neurons. Here we report […]
Abstract Axons in the developing nervous system are directed via guidance cues, whose expression varies both spatially and temporally, to create functional neural circuits. Existing methods to create patterns of neural connectivity in vitro use only static geometries, and are unable to dynamically alter the guidance cues imparted on the cells. We introduce the use of AC electrokinetics to […]