NeurofluidicsTM approach enables continuous electrophysiological recordings providing data to evaluate neuronal functional activity. It allows to investigate a compound’s impact on functional activity and recovery, synapse formation or network dynamics. A network can be monitored within all types of microfluidics architecture, including compartmentalized devices. Such approach allows the constant monitoring of an entire network while applying cells or compounds on one compartement only. Electrophysiological data are recorded using Multi Electrodes Arrays (MEA) and analyzed with internally developped softwares for network parameters extraction under different conditions.