Neurofluidic devices are ideally suited for high-resolution axonal transport studies. The microchannel technology enables a fluidic isolation between compartments. The measurement of retrograde transport kinetics is done simultaneously on a large number of axons. The Asymetric Tri-Compartment device is perfectly suited to conduct high-resolution vesicular transport studies. Co-culture of different neuronal types allows the creation of synaptic compartment to reproduce physiological conditions.