Organ-on-chip

Organ-on-chip technologies use microfluidic devices to reproduce the function of a given organ. Initially developed by the Ingber lab, multiple laboratories have developed specific organs or multiple organs on chip:

References :
D. Huh, B. D. Matthews, A. Mammoto, M. Montoya-Zavala, H. Y. Hsin, D. E. Ingber, Science (80-. ). 2010, 328.

F. Zheng, F. Fu, Y. Cheng, C. Wang, Y. Zhao, Z. Gu, Small 2016, 12, 2253.

extracted from Zheng et al., Copyright Small

Neurofluidics™

Neurofluidics™ chips are microfluidic devices specially designed and fabricated for neuroscience applications. This neuro engineering technology allows fine control of fluid flow in a confined environment, thus allowing the control deposition of cells, in 2D or in 3D. The compartment can be connected by microchannels.

Several scientific publications have been published over the past decades to demonstrate the full potential of Neurofluidics™ chips.

References :
A. M. Taylor, M. Blurton-Jones, S. W. Rhee, D. H. Cribbs, C. W. Cotman, N. L. Jeon, Nat Meth 2005, 2, 599.

J. W. Park, B. Vahidi, A. M. Taylor, S. W. Rhee, N. L. Jeon, Nat. Protoc. 2006, 1, 128.
A. M. Taylor, N. L. Jeon, Curr. Opin. Neurobiol. 2010, 20, 640.
F. Difato, L. Schibalsky, F. Benfenati, A. Blau, Int. J. Optomechatronics 2011, 5, 191.

deposition chambers

NETRI has developed a unique technology to control the deposition of neurons within microfluidic chambers.

Article soon to be available

connectome on chip

Network analysis is growing as an approach to model the complexity of the human brain. Sporns et al. have shown that among primate connectomes, regular and small (3, 4 or 5 nodes) structural motifs form characteristic network building blocks that, when computationally assembled, resemble real brain networks, including small-world attributes. The in vitro reproduction of highly simplified models of in vivo networks with increased complexity will help in understanding the structure-function relationship and will in fine serve as minimalist reproductions of part of the human connectome, which could then be used to create high-throughput synthetic models of neurodegenerative diseases, high-throughput assays for drug discovery when coupled with physiologically relevant cells, and to create biological neuronal computers on chip.

Using Neurofluidics™ devices, NETRI has reconstructed both, the structural and functional network in vitro.

References :
O. Sporns, R. Kötter, PLoS Biol 2004, 2, e369.

J. W. Park, H. J. Kim, M. W. Kang, N. L. Jeon, Lab Chip 2013, 13, 509.
D. Huh, G. A. Hamilton, D. E. Ingber, Trends Cell Biol 2011, 21, 745.
O. Feinerman, A. Rotem, E. Moses, Nat Phys 2008, 4, 967.
T. Honegger, M. I. Thielen, S. Feizi, N. E. Sanjana, J. Voldman, Sci. Rep. 2016, 6, 28384.