NeuroInflammation Model

Neurofluidics™ devices can replicate the neuroinflammation process using injury methods (as Amyloid beta or alpa-synuclein). The evaluation of the neuroprotective effect of a compound is quantified by measuring the localization of glial cells regarding protected neurons or not within the same culture. By using large microchannels between chambers, gradients of inflammatory molecules provide chemiotaxis routes to glial cells.

Prion Spreading Model

Parkinson’s disease is characterized by the loss of dopaminergic neurons in specific brain regions. Our basal ganglia loop-on-chip provides a unique in vitro solution to study the different mechanisms of prion-like spreading molecules. Five connected compartments are created using the 3D-Deposition Chamber technology while maintaining their in vivo counterparts cell density ratios and directionality patterns. Deposition chamber technology allows to translate the in vivo basal ganglia loop (in direct way) involved in Parkinson’s disease into a unique in vitro architecture. A-synuclein can be applied only in the “substantia nigra” node while maintaining fluid isolation and functional activity with the other compartment.

Pain Model

The pain model involves the spinothalamic tract composed of the dorsal root ganglion (DRG), the dorsal horn of the spinal cord and the thalamus. Neurofluidics™ chips reproduce such connectivity pattern by isolating and connecting each region. An analgesic can be applied on the dorsal root ganglion only after an axotomy injury to study its impact on the activity of the spinothalamic tract. The tri-compartment explant device allows the fine placement of a DRG using deposition chamber technology and growth of axons with extensive microgrooves. Axotomy can be performed in the central channel on axons only and follow their growth kinetics and Schwann cells proliferation. Coupling the device with microelectrode arrays (MEA) to perform simultaneously functional recording of pain markers

Mucous Innervation

This product range combines microchannels and membranes technologies which allows innervation of any types of epithelial cells growing in 3D. We are currently designing a human sensory neurons innervating nasal mucosa for Covid-19 disease modelling in order to understand the mechanism of SARS-CoV-2 Central Nervous System penetration.

Gut Brain axis

Gut-brain interactions are implicated in several physiological mechanisms concerning toxicology of nutrition compounds, nutrition pathologies, depression or treatment related to microbiota dysfunction. This project aims at coupling, on a unique chip, a microbiota compartment connected to vagual neurons via two barriers, intestin epithelium and Blood Brain Barrier. Combining both NETRI’s hight throughput technologies and electrical activity recording, we hope to accelerate the microbiota related industry in assessing new treatments.