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Humanization

The use of human cells in NETRI’s microfluidic devices offers the possibility to industrialize and standardize in-vitro human predictive models. NETRI’s microfluidic devices allow for fewer cells and pharmacological compounds while controlling flow in a relevant and minimalist environment with a versatile architecture. NETRI has developed a standardized and reproducible method to characterize five different human neural types, keratinocytes, embryoid bodies and muscle.

Humanization

The use of human cells in NETRI’s microfluidic devices offers the possibility to industrialize and standardize in-vitro human predictive models. NETRI’s microfluidic devices allow for fewer cells and pharmacological compounds while controlling flow in a relevant and minimalist environment with a versatile architecture. NETRI has developed a standardized and reproducible method to characterize five different human neural types, keratinocytes, embryoid bodies and muscle.

Growth Kinetics

Growth kinetics can be assessed by the triangular design of our DuaLink Delta microfluidic devices. This specific architecture allows the accurate monitoring of neurite growth kinetics in a neuronal culture thanks to the different lenght of microchannels. The device permits to measure the maximum achievable length of projecting neurites throughout microchannels over time and to report variations in neurite length under several conditions. Coupled to our analytical software, the chip allows a precise characterization of neurite length dynamics.

Growth Kinetics

Growth kinetics can be assessed by the triangular design of our DuaLink Delta microfluidic devices. This specific architecture allows the accurate monitoring of neurite growth kinetics in a neuronal culture thanks to the different lenght of microchannels. The device permits to measure the maximum achievable length of projecting neurites throughout microchannels over time and to report variations in neurite length under several conditions. Coupled to our analytical software, the chip allows a precise characterization of neurite length dynamics.

Axonal Transport

Neurofluidic devices are ideally suited for high-resolution axonal transport studies. The Microchannels technology enables a fluidic isolation between compartments. The measurement of retrograde transport kinetics is done simultaneously on a large number of axons. The DuaLink, DuaLink Shift and TriaLink devices are perfectly suited to conduct high-resolution vesicular transport studies. Co-culture of different neuronal types allows the creation of compartments to reproduce physiological conditions.

Axonal Transport

Neurofluidic devices are ideally suited for high-resolution axonal transport studies. The Microchannels technology enables a fluidic isolation between compartments. The measurement of retrograde transport kinetics is done simultaneously on a large number of axons. The DuaLink, DuaLink Shift and TriaLink devices are perfectly suited to conduct high-resolution vesicular transport studies. Co-culture of different neuronal types allows the creation of compartments to reproduce physiological conditions.

Organoids

Cultivating organoids in chip requires a significant amount of space, while compartimentalized, for them to pursue their growth under constant perfusion. A unique 3D-Deposition Chamber has been specially design to accept organoids up to 500 µm before seeding and to perfuse growth media for them to grow as big as 4 mm in diameter. Using the 3D embedded inlet and oulet channels and the seed flow control, an organoid can be placed directly on MEA/HDMEA. The NeoBentoTM can accept up to 48 organoids at the same time during several months and perform mutliplexed experiments.

Organoids

Cultivating organoids in chip requires a significant amount of space, while compartimentalized, for them to pursue their growth under constant perfusion. A unique 3D-Deposition Chamber has been specially design to accept organoids up to 500 µm before seeding and to perfuse growth media for them to grow as big as 4 mm in diameter. Using the 3D embedded inlet and oulet channels and the seed flow control, an organoid can be placed directly on MEA/HDMEA. The NeoBentoTM can accept up to 48 organoids at the same time during several months and perform mutliplexed experiments.

Network Activity

NETRI’s MEA compatibility approach enables continuous electrophysiological recordings providing data to evaluate neuronal functional activity. It allows to investigate a compound’s impact on functional activity and recovery, synapse formation or network dynamics. A network can be monitored within all types of NETRI’s microfluidics architecture, including compartmentalized devices. Such approach allows the constant monitoring of an entire network while applying cells or compounds on one compartement only. Electrophysiological data are recorded using Multi Electrodes Arrays (MEA) and analyzed with internally developped softwares for network parameters extraction under different conditions.

Network Activity

NETRI’s MEA compatibility approach enables continuous electrophysiological recordings providing data to evaluate neuronal functional activity. It allows to investigate a compound’s impact on functional activity and recovery, synapse formation or network dynamics. A network can be monitored within all types of NETRI’s microfluidics architecture, including compartmentalized devices. Such approach allows the constant monitoring of an entire network while applying cells or compounds on one compartement only. Electrophysiological data are recorded using Multi Electrodes Arrays (MEA) and analyzed with internally developped softwares for network parameters extraction under different conditions.

Viral & Protein Propagation

Compartmentalized devices allows to fluidically isolate two or more cellular populations, even when connected by microchannels. When using neurons, geometrical constraints due to microchannels allows neurites to grow and create functionnal synapses with distal neurons, both in a unidirectional or bidirectional way. Thanks to the fluidic isolation between compartments, a virus or protein can be applied to one cell population only, in an acute or chronic perfusion. Internalized molecules can be monitored for retrograde or anterograde transport, both by monitoring their presence on the remote compartment or within the axons themselves. Microchannels geometries can also be adapted to create gradients of secreted molecules or to allow migrating cells to pass from one compartment to the other.

Viral & Protein Propagation

Compartmentalized devices allows to fluidically isolate two or more cellular populations, even when connected by microchannels. When using neurons, geometrical constraints due to microchannels allows neurites to grow and create functionnal synapses with distal neurons, both in a unidirectional or bidirectional way. Thanks to the fluidic isolation between compartments, a virus or protein can be applied to one cell population only, in an acute or chronic perfusion. Internalized molecules can be monitored for retrograde or anterograde transport, both by monitoring their presence on the remote compartment or within the axons themselves. Microchannels geometries can also be adapted to create gradients of secreted molecules or to allow migrating cells to pass from one compartment to the other.

Synaptic Toxicity

Asymetric tri-compartment architecture of DuaLink Shift microfluidic devices allow the reconstitution of a neural network between two neural populations connected through a fluidically isolated synaptic chamber. Thanks to the asymetry of the connecting microchannels, axons from one population connect to dendrites from the other, with a high probability, only in the middle compartment. The use of multiplexed microchannels offers also the possibility to study subcellular trafficking while altering synapses. Coupled to MEA, the device can be used to monitor the electrophysiological impact of a compound when applied in the synaptic chamber only. Alternatively, by seeding cells in the middle compartment when a neural network is matured, interactions between several type of cells and synapses can be monitored.

Synaptic Toxicity

Asymetric tri-compartment architecture of DuaLink Shift microfluidic devices allow the reconstitution of a neural network between two neural populations connected through a fluidically isolated synaptic chamber. Thanks to the asymetry of the connecting microchannels, axons from one population connect to dendrites from the other, with a high probability, only in the middle compartment. The use of multiplexed microchannels offers also the possibility to study subcellular trafficking while altering synapses. Coupled to MEA, the device can be used to monitor the electrophysiological impact of a compound when applied in the synaptic chamber only. Alternatively, by seeding cells in the middle compartment when a neural network is matured, interactions between several type of cells and synapses can be monitored.