Organs on chips technologies use microfluidic devices to reproduce the function of a given organ. Initially developed by the Ingber lab , multiple labs have developed organ specific or multiple organs on chips:
D. Huh, B. D. Matthews, A. Mammoto, M. Montoya-Zavala, H. Y. Hsin, D. E. Ingber, Science (80-. ). 2010, 328.
F. Zheng, F. Fu, Y. Cheng, C. Wang, Y. Zhao, Z. Gu, Small 2016, 12, 2253.
extracted from Zheng et al., Copyright Small
Neurofluidics™ chips are microfluidic devices specially designed and fabricated for neuroscience applications. Microfluidic technologies allow to fine control fluid flow in (A) confined environment, thus allowing the control deposition of cells, in 2D or in (pas d’article) 3D. allowing the control of the deposition of cells ? (the )Compartment can be connected by microchannels.
Several scientific publications have been published other the past decades to demonstrate to full potential of neurofluidic TM chips:
A. M. Taylor, M. Blurton-Jones, S. W. Rhee, D. H. Cribbs, C. W. Cotman, N. L. Jeon, Nat Meth 2005, 2, 599.
J. W. Park, B. Vahidi, A. M. Taylor, S. W. Rhee, N. L. Jeon, Nat. Protoc. 2006, 1, 128.
A. M. Taylor, N. L. Jeon, Curr. Opin. Neurobiol. 2010, 20, 640.
F. Difato, L. Schibalsky, F. Benfenati, A. Blau, Int. J. Optomechatronics 2011, 5, 191.
NETRI has developed a unique technology to control the deposition of neurons within microfluidic chambers.
Paper soon to be available
Network analysis is growing as an approach to model the complexity of the human brain. Sporns et al. have shown that among the connectomes of primates, regular and small (3, 4 or 5 nodes) structural motifs form characteristic network building blocks that, when assembled computationally, resemble real brain networks, including small-world attributes. Reproducing in vitro very simplified models of in vivo increased complexity networks will help understanding of the structure-function relationship and in fine serve as minimalistic reproductions of part of the human connectome, which could then be used to create high-throughput synthetic models of neurodegenerative diseases, high-throughput assays for drug discovery when coupled with physiologically relevant cells, and to create biological neuronal computers on-chip.
Using neurofluidic devices we have reconstructed both structural and function network in vitro.
O. Sporns, R. Kötter, PLoS Biol 2004, 2, e369.
J. W. Park, H. J. Kim, M. W. Kang, N. L. Jeon, Lab Chip 2013, 13, 509.
D. Huh, G. A. Hamilton, D. E. Ingber, Trends Cell Biol 2011, 21, 745.
O. Feinerman, A. Rotem, E. Moses, Nat Phys 2008, 4, 967.
T. Honegger, M. I. Thielen, S. Feizi, N. E. Sanjana, J. Voldman, Sci. Rep. 2016, 6, 28384.